| Alphabetic List of SBS Restriction Endonucleases |
|
|
| Restriction Endonucleases (A-B) |
|
| Alu I |
AG CT |
|
|
| Cat. # |
Size |
Conc. |
| 101-1 |
250 units |
8-12u/µl |
|
|
| Source: |
Arthrobacter luteus |
|
| Buffer supplied: |
10×L |
|
| Substrate for unit definition: |
Lambda DNA |
|
| Reaction conditions: |
10 mM Tris-HCl (pH 7.9, 25oC), 10 mM MgCl2,1 mM dithiothreitol,
100µg/ml BSA. Incubate at 37oC. |
|
|
| Storage buffer: |
100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol,200 µg/ml BSA and 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
Fifty units of Alu I do not produce any unspecific cleavage
products after 16 hrs incubation with 1 µg of Lambda DNA at 37oC.
After ten-fold overdigestion with Alu I, greater than 95% of
the DNA fragments can be ligated and recat with this enzyme. |
|
| Heat inactivation: |
65oC for 20 minutes. |
|
|
 |
|
| ApaL I |
GTGCAC |
|
|
| Cat. # |
Size |
Conc. |
| 148-1 |
200 units |
8-12u/µl |
|
|
| Source: |
Acetobacter pasteurianus(ATCC 12875) |
|
| Buffer supplied: |
10×L |
|
| Substrate for unit definition: |
Lambda DNA |
|
| Reaction conditions: |
10 mM Tris-HCl (pH 7.9,25oC),10 mM MgCl2,1 mM DTT,100 µg/ml,
BSA. Incubate at 37oC. |
|
|
| Storage buffer: |
50 mM KCl,10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM DTT, 200 µg /ml BSA, 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
100units of ApaL I do not produce any unspecific cleavage
products after 16 hrs incubation with 1 µg of Lambda DNA at 37oC.
After 100-fold overdigestion with ApaL I, greater than 98% of
the DNA fragments can be ligated and recut with this enzyme. |
|
| Heat inactivation: |
No. |
|
|
 |
|
| Asu II (BspT104 I) |
TTCGAA |
|
|
| Cat. # |
Size |
Conc. |
| 102-1 |
1,000 units |
8-12u/µl |
|
|
| Source: |
Actinobacillus suis |
|
| Buffer supplied: |
10×AsuII |
|
| Substrate for unit definition: |
Lambda DNA (Hind III digest) |
|
| Reaction conditions: |
50 mM NaCl, 10 mM Tris-HCl (pH 7.9, 25oC), 10 mM MgCl2, 1 mM ,
dithiothreitol, 0.1% Triton X-100, 100 µg/ml BSA. Incubate at 37oC.. |
|
|
| Storage buffer: |
100 mM KCl, 10 mM Tris-HCl (pH 7.9, 25oC), 0.1mM EDTA,
1 mM dithiothreitol, 0.15% Triton X-100, 200 µg /ml BSA and 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
Fifty units of Asu II do not produce any unspecific cleavage
products after 16 hrs incubation with 1µg of Lambda DNA at 37oC.
After 50-fold overdigestion with Asu II, greater than 95% of
the DNA fragments can be ligated and recat with this enzyme. |
|
| Heat inactivation: |
65oC for 20 minutes. |
|
|
 |
|
| BamH I |
GGATCC |
|
|
| Cat. # |
Size |
Conc. |
| 103-1 |
2,500 units |
8-12u/µl |
|
|
| Source: |
Bacillus amyloliquefaciens H |
|
| Buffer supplied: |
10×BamH I |
|
| Substrate for unit definition: |
Lambda DNA |
|
| Reaction conditions: |
100 mM NaCl, 10 mM Tris-HCl (pH 7.9, 25oC), 5 mM MgCl2, 1 mM dithiothreitol,
100 µg/ml BSA. Incubate at 37oC.. |
|
|
| Storage buffer: |
50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
Ten units of BamH I do not produce any unspecific cleavage
products after 16 hrs incubation with 1 µg of Lambda DNA at 37oC.
After ten-fold overdigestion with Alu I, greater than 95% of
the DNA fragments can be ligated and recat with this enzyme. |
|
| Heat inactivation: |
80oC for 20 minutes. |
|
| Star activity: |
Conditions of low ionic strength, high enzyme concentration
glycerol concentration>5% or pH>8.0 may result in star activity. |
|
|
 |
|
| Bcl I (Fba I) |
TGATCA |
|
|
| Cat. # |
Size |
Conc. |
| 104-1 |
500 units |
8-12u/µl |
|
|
| Source: |
Bacillus caldolyticus |
|
| Buffer supplied: |
10×M |
|
| Substrate for unit definition: |
Lambda DNA (dam-) |
|
| Reaction conditions: |
50 mM NaCl, 10 mM Tris-HCl (pH 7.9, 25oC), 10 mM MgCl2, 1 mM dithiothreitol,
100 µg/ml BSA. Incubate at 50oC under parafin oil. |
|
|
| Storage buffer: |
50 mM KCl, 10 mM Tris-HCl (pH. 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA, and 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
Fifty units of Bcl I do no produce any unspecific clevage
products after16 hrs incubation with 1 µg of Lamba DNA at 50oC.
After 50-fold overdigestion with Bcl I, greater than 95% of
the DNA fragments can be ligated and recut with this enzyme. |
|
| Heat inactivation: |
No |
|
| Star activity: |
Large excess of the enzyme results in the appearance of star activity. |
|
|
 |
|
| Bgl I |
GCCNNNNNGGC |
|
|
| Cat. # |
Size |
Conc. |
| 105-1 |
1000 units |
8-12u/µl |
|
|
| Source: |
Bacillus globigii lacking Bgl II |
|
| Buffer supplied: |
10×Bgl I |
|
| Substrate for unit definition: |
Lambda DNA |
|
| Reaction conditions: |
50 mM NaCl, 100 mM Tris-HCl (pH 7.9, 25oC), 5 mM MgCl2,
0.025% Triton X-100, 100 µg/ml BSA. Incubate at 37oC. |
|
|
| Storage buffer: |
200 mM NaCl, 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
Fifty units of Bgl do not produce any unspecific cleavage
products after 16 hrs incubation with 1 µg of Lambda DNA at 37oC.
After fifty-fold overdigestion with Blg I, greater than 95% of
the DNA fragments can be ligated and recat with this enzyme. |
|
| Heat inactivation: |
65oC for 20 minutes. |
|
|
 |
|
| Bgl II |
AGATCT |
|
|
| Cat. # |
Size |
Conc. |
| 106-1 |
1000 units |
8-12u/µl |
|
|
| Source: |
Bacillus globigii lacking Bgl I |
|
| Buffer supplied: |
10×H |
|
| Substrate for unit definition: |
Lambda DNA |
|
| Reaction conditions: |
100 mM NaCl, 50 mM Tris-HCl (pH 7.9, 25oC), 10 mM MgCl2,
1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37oC. |
|
|
| Storage buffer: |
50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
Fifty units of Bgl II do not produce any unspecific cleavage
products after 16 hrs incubation with 1 µg of Lambda DNA at 37oC.
After 50-fold overdigestion with Bgl II, greater than 95% of
the DNA fragments can be ligated and recat with this enzyme. |
|
| Heat inactivation: |
No. |
|
|
 |
|
| BseA I (BspM II, Aor13H I) |
TCCGGA |
|
|
| Cat. # |
Size |
Conc. |
| 107-1 |
500 units |
8-12u/µl |
|
|
| Source: |
Bacillus stearothermophilus |
|
| Buffer supplied: |
10×BseA I |
|
| Substrate for unit definition: |
Lambda DNA |
|
| Reaction conditions: |
100 mM NaCl, 10 mM Tris-HCl (pH 8.0, 25oC), 5 mM MgCl2,
1 mM dithiothreitol, 0.02% Triton X-100, 100 µg/ml BSA. Incubate at 55oC under parafin oil. |
|
|
| Storage buffer: |
50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 500 µg/ml BSA and 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
Fifty units of BseA I do not produce any unspecific cleavage
products after 16 hrs incubation with 1 µg of Lambda DNA at 55oC.
After 100-fold overdigestion with BseA I, greater than 98% of
the DNA fragments can be ligated and recut with this enzyme. |
|
| Heat inactivation: |
No. |
|
|
 |
|
| BseB I (BstN I) |
CC(A/T)GG |
|
|
| Cat. # |
Size |
Conc. |
| 108-1 |
1,500 units |
8-12u/µl |
|
|
| Source: |
Bacillus staerothermophilus |
|
| Buffer supplied: |
10×M |
|
| Substrate for unit definition: |
Lambda DNA |
|
| Reaction conditions: |
50 mM NaCl, 10 mM Tris-HCl (pH7.9, 25oC), 10 mM MgCl2,
1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 60oC under parafin oil. |
|
|
| Storage buffer: |
50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50% glycerol. Store at -20oC. |
|
|
| Absence of contaminants: |
Fifty units of BseB I do not produce any unspecific cleavage
products after 16 hrs incubation with 1 µg of Lambda DNA at 60oC.
After ten-fold overdigestion with BseB I, less than 50% of
the DNA fragments can be ligated. |
|
| Heat inactivation: |
No. |
|
| Star activity: |
Low salt concentration or large excess of enzyme results in
the appearance of star activity. |
|
| Note: |
BseB I cut DNA is difficult to ligate with T4 DNA Ligase.
Ligation is enhanced in the presence of 15% PEG4000. |
|
|
 |
|
| BseC I (Cla I) |
ATCGAT |
|
|
| Cat. # |
Size |
Conc. |
| 109-1 |
500 units |
8-12u/µl |
|
|
| Source: |
Bacillus stearothermophilus |
|
| Buffer supplied: |
10×H |
|
| Substrate for unit definition: |
Lambda DNA |
|
| Reaction conditions: |
100 mM NaCl, 50 mM Tris-HCl (pH 7.9, 25oC), 10 mM MgCl2,
1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 55oC under parafin oil. |
|
|
| Storage buffer: |
100 mM KCl, 10 mM Tris-HCl (pH 7.9, 25oC), 0.1 mM EDTA,
1 mM dithiothreitol, 0.15% Triton X-100, 200 µg/ml BSA and 50% glycerol. Store at - 20oC. |
|
|
| Absence of contaminants: |
Fifty units of BseC I do not produce any unspecific cleavage
products after 16 hrs incubation with 1 µg of Lambda DNA at 55oC.
After 100-fold overdigestion with BseC I greater than 95% of
the DNA fragments can be ligated and recut with this enzyme. |
|
| Heat inactivation: |
No. |
|
|
 |
|
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Viswagen Biotech Pvt. Ltd., 23/863G, Thazhathuveettil Buildings, Market Road, Pala, Kerala 686 575; India
|
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Web: www.viswagen.com, Phone: +91-98-46804374; Fax: +91-4822-215075
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Contacts: customer service: customer.service@viswagen.com
|
| Copyright © 2007 Viswagen Biotech Pvt. Ltd. |
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