Home  Careers  Contact Us About Viswagen Products Ordering Information Nucleic Acid Stain GoldViewTM Nucleic Acid Stain- An Alternative to EB Description: GoldView TM is a new, safe nucleic acid stain instead of tranditional ethidium bromide (EB) for detecting double- stranded DNA, single-stranded DNA, and RNA in agarose gel. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at approximately 290 nm and one at approximately 490nm. GoldView TM is as sensitive as EB, and its protocol is the same way to EB. Compared to EB known as strong mutagen, GoldView TM cause much fewer mutations is the Ames test. In addition, GoldView TM has a negative test in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. So it is wise to choose GoldView TM instead of EB for detecting nucleic acid in agarose gels. Cat. No. Volume HGV-1 1ml Note: 1ml of GoldView TM is sufficient for 20 L of agarose gel. Protocol: 1. Prepare 100 ml of agarose gel solution (concentration from 0.8~2.0%) in a 250 ml flask and mix it thoroughly. Place the flask in the microware, heat on high until the solution is completely clear and no small floating particles are visible (about 2~3 minutes). 2. Add 5 µl of GoldViewTM to the gel solution. Swirl the flask gently to mix the solution and avoid forming bubbles. 3. While the gel solution cools below 50?, pour it into the gel tray until the comb teeth are immersed about 1/4~1/2 into the gel solution. 4. Allow the agarose gel to cool until solidified. Load samples on the gel and perform electrophoresis. 5. Detect the bands under UV illumination. Note: 1. The thickness of gel should be less than 0.5cm since thick gels may decrease sensitivity. 2. Repeated melting of gels containing GoldViewTM may result in low sensitivity. 3. GoldViewTM allows visualization of DNA(>50ng) in the agarose gel under visible light. This eliminates the need for exposure to UV light, which can nick and damage DNA.The intact DNA fragments purified from agarose gel can increase the efficiency of subsequent molecular biology manipulations such as cloning transformation and transcription. 4. Although it is noncarcinogenic, GoldViewTM may irritate skin and eyes. Please wear gloves while handing Storage: Store at room temperature. The agarose gel indicates the sensitivity of GoldView is equivalent to thant of EB. Goldview™ is a registered trademark of SBS Genetech Co. Ltd. Viswagen Biotech Pvt. Ltd., 23/863G, Thazhathuveettil Buildings, Market Road, Pala, Kerala 686 575; India Web: www.viswagen.com, Phone: +91-98-46804374; Fax: +91-4822-215075 Contacts: customer service: customer.service@viswagen.com Copyright © 2007 Viswagen Biotech Pvt. Ltd.