Home  Careers  Contact Us About Viswagen Products Ordering Information PCR Related Products U-Taq DNA Polymerase Cat. No. Size EU-500 500u Description The Polymerase gene from Thermus aquaticus (strain YT1) is cloned and expressed in E. coli, then highly purified to produce U-Taq DNA polymerase. It is used in the amplification and sequence testing of DNA through PCR. The quality of the U-Taq DNA polymerase has been tested by the Activity Test, SDS-Page, Endonuclease Test, etc. Reaction Buffer (10X): 100 mM Tris-HCl, 400mM KCI, 15mM MgCl2 , pH9.0 Dilution Buffer: 20mL Tris-HCl, 100mM KCI, 0.5 mM EDTA, 1mM DTT, 0.5% Tween 20, 0.5 % IGEPAL CA-630(pH 8.0 Storage Buffer: 20 mM Tris-HCI, 0.5 mM EDTA, 1mM DTT, 0,5% Tween 20, 0.5% IGEPAL CA-630(pH 8.0.). Store at -20oC. Concentration: 5,000 units/mL. Involve the dNTPs mixture: The concentration of each dNTPs is 2.5 mM. Taq-redTM DNA Polymerase Cat. No. Size ER-500 500u Description Taq-red DNA Polymerase is an unique blend of U-Taq DNA Polymerase with an inert red dye. It offers the same performance as Taq DNA Polymerase . Several benefits are as following: This red dye enables quick visual confirmation of enzyme addition and reaction mixing, which makes performance more convenient. After PCR amplification, samples can be removed from the reaction and loaded directly onto agarose gel without the addition of loading buffer or tracking dye. The red dye, acting as a tracking dye, migrates between bromophenol blue and xylenecyanol at about the same rate as 400~500bp fragment. The concentration of Taq-red is only 1unit/µl, so the enzyme can be transferred and added more accurately. The enzyme generates PCR products with 3'-dA overhangs, suitable for T-A cloning. s-Pfu DNA Polymerase Cat. No. Size EP-500 500u Description s-Pfu DNA Polymerase is a thermostable enzyme with a molecular weight of 90 kDa. It catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction, resulting in blunt-ended PCR products without 3'-dA overhangs. s-Pfu DNA Polymerase exhibits 5'→3' exonuclease (proofreading) activity that enables the polymerase to correct the mis-incorporation of nucleotide, and lacks 5'→3' exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter than 3kb.The extension rate of s-Pfu DNA Polymerase is about 600bp/min in standard condition. The appropriate reaction temperature is 65oC~75oC, the working concentration of dNTPs is 100-300µM, the working concentration of Mg2+ is 2~3mM, and the optimal pH is 8.1~9.1. The amount of enzyme is 1~1.5unit for 20µl PCR reaction, while 2~3units for 50µl PCR reaction. Easy-DoTM PCR PreMix Description The Easy-DoTM PCR PreMix is a pre-mixed preparation in a lyophilized format. The mix contains U-Taq DNA polymerase, reaction buffer, dNTPs, and tracking dye for efficient PCR amplification. For reaction set-up, all you have to do is to add templates, specific primers and water. After PCR amplification, samples can be loaded directly onto agarose gel without the addition of tracking dye. The PCR premix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination. Storage: Store at -20oC for one year. Description and Size Cat. No. Quantity Easy-DoTM PCR PreMix, 0.2ml thin wall microtube, 20µl reaction EQ2.2-50 50 tubes Easy-DoTM PCR PreMix, 0.2ml thin wall microtube, 50µl reaction EQ5.2-50 50 tubes Easy-DoTM PCR PreMix, 0.5ml thin wall microtube, 20µl reaction EQ2.5-50 50 tubes Easy-DoTM PCR PreMix, 0.5ml thin wall microtube, 50µl reaction EQ5.5-50 50 tubes dNTPs Mix(10mM each) Description 10mM dNTPs Mix is a ready-to-use solution of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of 10mM each in sterile deionized water at pH7.5, whose purity is up to 99.5% (HPLC). It is free of RNase and DNase, and is suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis and nick translation. Storage: Store at -20oC. Cat. No. Size EN-1 1ml dNTPs in Separate Tube(100mM each) Description dNTPs in Separate Tube contains 4×0.4ml of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of 100mM each in sterile deionized water at pH7.5, whose purity is up to 99.5% (HPLC). It is free of RNase and DNase, and suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis and nick translation. Storage: Store at -20oC Cat. No. Size EN-2 4×0.4ml PCR enhancer Description PCR enhancer can improve the efficiency of PCR amplification, increase the specificity of PCR products, and reduce non-specific and undesirable PCR products. The recommended amount is 1/10 of reaction volume. Cat. No. Size EE-1 1ml PCR Optimizer Description GC-rich regions may form rigid secondary structure which makes difficult or impossible for the DNA polymerases to work under standard PCR conditions. The PCR optimizer can optimize PCR of problematic or GC-rich templates. The recommended amount is 1/5 of reaction volume. Cat. No. Size EO-1 1ml Viswagen Biotech Pvt. Ltd., 23/863G, Thazhathuveettil Buildings, Market Road, Pala, Kerala 686 575; India Web: www.viswagen.com, Phone: +91-98-46804374; Fax: +91-4822-215075 Contacts: customer service: customer.service@viswagen.com Copyright © 2007 Viswagen Biotech Pvt. Ltd.