Home  Careers  Contact Us About Viswagen Products Ordering Information Protein Related Products PRO-STAINTM Prestained Protein Marker I Cat. No. Quantity PPM-125 125µl Description PRO-STAINTM Prestained Protein Marker I for Broad Range is a mixture of purified proteins covalently coupled to a blue and violet dye that resolved to 9 bands between 9 and 198kDa when electrophoresed. The product contain high-concentration of PRO-STAINTM Prestained Protein MarkerI for Brand Range, therefore very little amount is necessary of them. It designed for monitoring of protein separation during SDS-PAGE, verifying western blot transfer efficiency, perfect for CBB staining, silver staining and negative staining. Color remains the same even after blotting. PRO-STAINTM Prestained Protein MarkerI is supplied in gel loading buffer and is ready-to-use. Storage: Store at -20oC, the quaranteed date is 2yr. Must be aliquot the protein marker in several tube, if more than stable. Characteristics: The separation state of sample can be presumed during electrophoresis. Contain high-concentration of pre-stained protein, therefore very little amount is necessary of them. Very good lot-to-lot consistency. Perfect for CBB staining, silver staining and negative staining. Ready-to-use. Storage Buffer: 3(W/V)% SDS 10mM DTT 2mM EDTA•2Na 0.01(W/V)%NaN3 10mM Tris-HCL (pH 6.8) 33(V/V)% Glycerol 8-20% SDS-PAGE Blot Apply 5µl on mini-gel and electrophoresed for 1hr at 15% SDS-PAGE gel. After electrophoresed, transfer for 2hr at 80 volt PRO-STAINTM Prestained Protein Marker II Cat. No. Quantity PPM-125 125µl Description PRO-STAINTM Prestained Protein Marker II is a mixture of purified proteins covalently coupled to a blue and violet dye that resolved to 6 bands between 19.3 and 116kDa when electrophoresed. The product contain high-concentration of PRO-STAINTM Prestained Protein Marker II therefore little amount is necessary of them. It designed for monitoring of protein separation during SDS-PAGE, verifying western blot transfer efficiency, perfect for CBB staining, silver staining and negative staining. Color remains the same even after blotting. PRO-STAINTM Prestained Protein Marker is supplied in gel loading buffer and is ready-to-use. Storage: Store at -20oC, the quaranteed date is 2yr. Must be aliquot the protein marker in several tube, if more than stable. Characteristics: The separation state of sample can be presumed during electrophoresis. Contain high-concentration of pre-stained protein, therefore very little amount is necessary of them. Very good lot-to-lot consistency. Perfect for CBB staining, silver staining and negative staining. Ready-to-use. Storage Buffer: 3(W/V)% SDS 10mM DTT 2mM EDTA•2Na 0.01(W/V)%NaN3 10mM Tris-HCL (pH 6.8) 33(V/V)% Glycerol 8-20% SDS-PAGE Blot Apply 5µl on mini-gel and electrophoresed for 1hr at 15% Gradient SDS-PAGE gel. After electrophoresed, transfer for 2hr at 80 volt. PRO-PREPTM protein Extraction Solution Cat. No. Quantity PES-50 50ml Description By using PRO-PREPTM,proteins can be simply extracted from all kinds of cells and tissues. The kit contains 5 kinds of protease inhibitors so it is possible to extract very highly purified proteins. Characteristics: When extraction proteins from cells or tissues, one doesn't necessarily apply appendix treatment. Able to minimize protein extraction time into 20-30 minutes. Protein stabilization buffer can make protein stable. There is no protein degradation due to freezing or thawing since there is no freezing at -20oC Extracted proteins are stable for more than 6 months when kept in -20oC. There is no absorbable error because there is no absorbable hindrances of PRO-PREP solution when measuring protein concentration. Very useful for protein separation in Western blot analysis because lonic detergent turns protein into monomers. Very useful for protein molecular weight analysis because denature protein into a monomers. Protein degradation is minimized by adding commonly used protease inhibitor and doesn't necessarily need to prepare protease inhibitor. SDS-PAGE and Western Blot Analysis Total protein was isolated from SNU601 and K562 cells using PRO-PREP kit. The isolated protein was electrophoretically analyzed on a 4%-12% PAGE gel and stained with Coomassie Brilliant dye. Western blot analysis was performed for the expression of CDK2 protein in cancer cell lines. PRO-MEASURE Protein Measurement Solution Cat. No. Quantity PMS-50 50ml Description Protein quantification is one of the important steps in protein research such as Western blot assay. Protein assay methods are diverse, and there is one which absorbs at 280nm without special reagents or operation. This method is simple, yet it also has a shortcoming not being able to analyze protein assay when no amino acid such as phenylalanine, tryptophan or tyrosine are present. Neither will it be used commonly due to its DNA absorbance interference (AI). The other methods are Lowry assay or BCA assay, which are highly sensitive for protein quantification, but is quite inconvenient and time-consuming. Contrarily, Bradford method is the most commonly used one (within 10min), which can detect the minimum amount of protein and is very simple to use. This PRO-MEASURE is even more convenient than Bradford assay, declining AI of solution itself so that the background absorbance is maintained very low. Characteristics: Very simple usage steps Short measurement time within 10min High sensitivity to detect 10-25ng/ml protein Lower background interference Able to quantify protein by minimum amount of reagents Contents: PRO-MEASURE solution (10×) 50ml Standard solution (BSA, 1mg/ml) 0.5ml Storage: Store at 4oC, and then stable for more than 1 year. EXPERIMENTAL INFORMATION Standard Curve: A dilution series of BSA protein was prepared containing 33.5µg, 22.4µg, 10.1µg, 6.8µg, 3µg, and 1µg. The protein was detected using standard protocols and then read at 595nm.The standard equation was generated by the standard curve. Comparison of Calculated with Actual Concentration for the BSA Test Samples Fidelity of Standard Equation Test Sample Actual concentration (µg) Calculated by the Standard Equation (µg) 1 25 25.7 2 15 14.3 3 5 5.15 The protein concentration calculated by the standard equation was compared with the actual concentration of protein. This relationship can be used for the accurate quantification of protein by PRO-MEASURE Kit. WEST-ZOL® [plus] Western Blot Detection System Cat. No. Quantity WZBD-100 100ml Description WEST-ZOL® [plus] Western Blot Detection System is a light emitting non-radioactive method for detection of immobilized specific antigens thorough horseradish peroxidase-labeled antibodies. The HRP(horseradish peroxidase) bound to an antibody induce chemilluminescence which is detected on X-ray film.Principles of WEST-ZOL® [plus] is that hydroxide ion (OH-) generated by horseradish preoxidase in aprotic media result in transition of luminol to 3'-aminophthalate, which induce emission of 425-510nm light. The X-ray film exposed to the light show a specific protein band.The WEST-ZOL® [plus] has greater sensitivity and longer signal duration than WEST-ZOL®. Characteristics: Simple step: WEST-ZOL® [plus] has very simple step, which consist of reaction detection solution and membrane bolt and exposure to X-ray film. Rapid reaction time: Specific protein detection may be achieved in less than 1-10 minute. High sensitivity: WEST-ZOL® [plus] is able to detection less than 1-2pg of antigen on membrane blot, at least 8-15x more sensitive than WEST-ZOL® . High resolution: WEST-ZOL® [plus] generate high contrast signal. Long duration time: Signal duration time of WEST-ZOL® [plus] is 7-15x longer than that of WEST-ZOL® [plus]. Storage: Store at 4oC, and then stable for more than 1 year. Kit Contents: Solution A: WEST-ZOL® [plus] Substrate Solution 50ml Solution B: WEST-ZOL® [plus] Enhancer Solution 50ml EXPERIMENTAL INFORMATION Leading Competitor and WEST-ZOL® [plus] WEST-ZOL® [plus] kit was compared with Leading Competitor in the signal sensitivity. The comparison at same conditions showed that the sensitity of WEST-ZOL® [plus] kits was excellent to Leading Competitor. Figure1. Comparison of Band Intensities Generated by Leading Competitor and WEST-ZOL® [plus] The duration time of Leading Competitor was determined by using HRP-tagged secondary antibody. After adding the Leading Competitor solution to the antibody, the fluorescent emission was figured by the time table. The reaction time was very fast (within 5 sec), and the emission was maintained for 5-10min.Signal Duration Time We have analyzed the signal duration time of WEST-ZOL® [plus] Kit. After adding the mixture of A and B solution into the HRP-tagged secondary antibody, the fluorescent signal was analyzed during the incubation at dark room. Figure2. Duration time of WEST-ZOL® [plus] Kit The duration time of WEST-ZOL® [plus] kit was determined by using HRP-tagged secondary antibody. After adding the WEST-ZOL® [plus] solution to the antibody, the fluorescent emission was figured by the time table. The reaction time was very fast (within 5 sec), and the emission was maintained for more than 30min. WEST-oneTM Western Blot Detection System Cat. No. Quantity WOBD-100 100ml Description Principles of WEST-oneTM Kit is that hydroxide ion (OH-) generated by horseradish peroxidase in aprotic media result in transition of luminol to 3'-aminophthalate, which induce emission of 425-510nm light. The X-ray film exposed to the light show a specific protein band.WEST-oneTM Western Blot Detection System is a light emitting non-radioactive method for detection of immobilized specific antigens thorough horseradish peroxidase-labeled antibodies. The HRP(horseradish peroxidase) bound to an antibody induce chemillum inescence which is detected on X-ray film.WEST-oneTM Western Blot Detection System is quite different most of western blot detection system, which consist of substrate solution and enhancer solution. WEST-oneTM Kit is consisted of just one bottle and spray type, which is very simple method of detection system. The most remarkable characteristics of the WEST-oneTM Kit is: there is no mix (substrate solution and enhancer solution). Characteristics: Spray type: Spray the WEST-oneTM Kit on the membrane. It has very simple step, which consist of reaction detection solution and membrane blot and exposure to X-ray film. Rapid reaction time: Specific protein detection may be achieved in less than 1-10 minute. High sensitivity: WEST-oneTM Kit is able to detect less than 1-2pg of antigen on membrane blot. High resolution: WEST-oneTM Kit generate high contrast signal. Long duration time. Storage: Store at 4oC in dark room, and then stable for more than 1 year. Kit Contents: WEST-oneTM Detection Solution 100ml All the above products are the registered trademark of SBS Genetech Co. Ltd. Viswagen Biotech Pvt. Ltd., 23/863G, Thazhathuveettil Buildings, Market Road, Pala, Kerala 686 575; India Web: www.viswagen.com, Phone: +91-98-46804374; Fax: +91-4822-215075 Contacts: customer service: customer.service@viswagen.com Copyright © 2007 Viswagen Biotech Pvt. Ltd.