Home  Careers  Contact Us About Viswagen Products Ordering Information RNA Related Products Oligo dT-Cellulose Cat. No. Size FOT-250 250mg Description Oligo dT-Cellulose is used for the isolation of poly(A)+ RNA. It consists of thymidylic acid oligomers of up to 30 nucleotides in length, covalently coupled to a cellulose matrix. It is free of RNase and DNase, and can be stored at 4oC. Poly rA Binding Assay: A Spectrophotometric determination of the amount of Poly rA (M.W.>100,000) bound per gram of cellulose. Poly rA binding capacity: 90A260ODs/gram. Redzol Reagent (for total RNA isolation) Cat. No. Size FTR-25 25ml Description Redzol Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method developed by Chomcyznski and Sacchi. During sample homogenization or lysis, Redzol Reagent maintains the integrity of the RNA, while disrupts cells and dissolvs cell components. The addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After the transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. This technique performs well with small quantities of tissue (50~100mg) and cells (5×106), and large quantities of tissue(≥1g)and cells(>107), of human, animal, plant, or bacterial origin. The simplicity of the Redzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA isolated by Redzol Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, and molecular cloning. For use in the polymerase chain reaction(PCR), treatment of the isolated RNA with amplification grade Dnase I is recommended when the two primers lie within a single exon. Redzol Reagent facilitates isolation of a variety of RNA species of large or small molecular size. For example, RNA isolated from rat liver, electrophoresed on an agarose gel, and stained with ethidium bromide, shows discrete bands of high molecular weight RNA between 7 kb and 15 kb in size, (composed of mRNA's and hnRNA's ) two predominant ribosomal RNA bands at -5kb (28S) and at -2 kb (18S) ,and low molecular weight RNA between 0.1 and 0.3kb (tRNA,5S). The isolated RNA has an A260/280 ratio of 1.6~1.8 when diluted into distilled water. The expected yield of RNA per mg of tissue is: liver and spleen, 6~10µg; kidney, 3~4µg; skeletal muscles and brain,1~5µg; placenta,1~4µg. The expected yield of RNA from 1×106 cultured cells is : epithelial cells, 8~15µg, fibroblasts, 5~7µg. Storage: Redzol has demonstrated stability of 12 months when stored at RT. However, we recommend storage at 2~8oC for optimal performance. DEPC (diethylpyrocarbonate) Cat. No. Quantity FDP-100 10ml Description Molecular formula C6H10O5, FW 162.1. min. 97%(NMR) Inactivates RNase in solution at about 0.1%(v/v), thus protecting RNA against degradation. Modification reagent for His and Tyr residues in proteins. Moisture causes hydrolysis to ethanol and carbon dioxide; pressure may build up in the package. DEPC is a solution for eliminating RNase from the surface of glass or plastic labware, DEPC can be removed by autoclaving. Unwanted RNase contamination is then eliminated. Storage:Store at 4oC RNase inhibitor Description RNase inhibitor is a ribonuclease inhibitor extracted from human placenta with a molecular weight 51kDa. It inhibits the activity of RNase by specially binding up to RNase with a non-covalent bond. RNase inhibitor, free of RNase or Nickase, can maintain its activity at pH from 5 to 8, and the highest one at pH7.8. The concentration of RNase inhibitor is 40units/µl. Storage:Store at -20oC. Cat. No. Quantity FRN-1k 1,000u M-MLV RTase (Moloney Murine Leukemia Virus Reverse Transcriptase) Description Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is a RNA-dependent DNA polymerase. This enzyme is able to use RNA molecule as a template and synthesize single-strand DNA. It is isolated from E.coli strain containing a recombinant clone with a subunit of 71kDa. Comparing to AMV Reverse Transcriptase, M-MLV RTase has a lower RNase H activity. It can be applied in synthesis of cDNA from RNA molecules. The concentration of M-MLV RTase is 200 units/µl. Storage: Store at -20oC Cat. No. Quantity FMM-10k 10,000u AMV Reverse Transcriptase Description AMV Reverse Transcriptase (AMV RTase) catalyzes the polymerization of DNA using RNA molecule as a template. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. AMV Reverse Transcriptase is used chiefly to transcribe mRNA into cDNA, which can then be cloned into vectors or amplified by PCR. It may also be used for filling in and labeling the 3'-termini of DNA with 5'-protruding ends. AMV Reverse Transcriptase can also be used for dideoxy DNA sequencing and RNA sequencing. Cat. No. Quantity FAM-500 500u Total RNA Isolation Kit Description The Total RNA Isolation Kit provides a fast and simple technique for the preparation of purified and intact total RNA from tissues and cultured cells in as little as 45 minute, depending on the number of samples to be processed. 50~100mg of tissue or 5~10×106 cultured cells can be processed per purification, depending on the type, function and RNA expression levels of the tissue. The purity and integrity of RNA isolated from tissue or cultured cells with the Total RNA Isolation Kit can meet the demands such as reverse transcription PCR (RT-PCR), RNase protection assays, primer extension, Northern blot analysis, oligo(dT) selection of poly(A)+ RNA, in vitro translation and cDNA library construction Cat. No. Size FTRI-25 25T Total RNA was isolated from Soybean using Redzol and using Total RNA Isolation Kit Lane 1-2 Total RNA was isolated from Soybean using Redzol. Lane 3-5 Total RNA was isolated from Soybean using Total RNA Isolation Kit. First-Strand cDNA Synthesis Kit Description The Kit is a complete system for efficient first-strand cDNA synthesis from RNA templates. First-Strand cDNA Synthesis Kit uses M-MLV Reverse Transcriptase, which exhibits lower RNase H activity compared to AMV Reverse Transcriptase. The Kit contains oligo(dT)18 and random nonamer primers (dN)9. Oligo(dT)18 is designed to prime selectively on mRNA with a poly(A)+ tail. A random nonamer can be used for the generation of cDNA libraries, to copy mRNAs that do not possess a poly(A)+ tail, or for synthesis of the 5'-end regions of mRNAs. The cDNA synthesized using this system can be used directly in PCR amplification. Cat. No. Size FFS-25 25T Two-Step RT-PCR Kit Description The Two-Step RT-PCR Kit has all the components required to perform first-strand cDNA synthesis followed by amplification of the cDNA product using a two-step process. In the first step, M-MLV Reverse Transcriptase is used to synthesize a DNA copy of input RNA. M-MLV RTase is a frequent choice for cDNA synthesis because of its ease of use and low intrinsic RNase activity. Oligo(dT)18 primers direct synthesis of a complementary strand of poly(A)+ mRNA. Random nonamer primers direct synthesis of sequences complementary to all RNA present in the reaction.In the PCR step, a portion of the cDNA product is amplified using U-Taq DNA polymerase and input primers. U-Taq polymerase, buffer, dNTPs are included in this kit. Key Features: Optimized for high performance using M-MLV Reverse Transcriptase and U-Taq DNA polymerase.Choice of priming methods: oligo(dT)18, random nonamer or gene-specific primers.Amplifies targets up to 4 kb. Cat. No. Size FTRP-25 25T RT-PCR amplification of tatol RNA using First Strand cDNA Synthesis Kit and Two-Step M-MLV RT-PCR Kit Lane1-4 Synthesis and amplification using the First Strand cDNA Synthesis Kit. Lane 5-8 Amplification using Two-Step M-MLV RT-PCR Kit. One-Step RT-PCR Kit Description The One-Step RT-PCR Kit is designed for optimal convenience in carrying out highly sensitive and specific RT-PCR in a single tube. One-step RT-PCR is a variation on standard (two-step) RT-PCR in which all components of the RT and PCR are mixed in one tube prior to starting the reactions and in which RT and PCR are thus carried out sequentially in one tube. This approach offers tremendous convenience when applied to analysis of single targets from multiple samples of RNA. This approach also minimizes the possibility for introduction of contaminants into reactions between the RT and PCR steps, since the RT and PCR reactions are carried out sequentially, without opening of reaction tubes between steps. The kit is composed of high quality reagents. The AMV reverse transcriptase is used for RT, and the optimized U-Taq polymerase is used for PCR. A unique buffer, 10×RT-PCR buffer suitable for both the RT and PCR steps and crucial for successful one-step RT-PCR, is included, along with dNTPs mixture and RNase inhibitor. DEPC-treated water is also provided. Use of this kit and the accompanying protocol will ensure excellent results. Cat. No. Size FORP-25 25T Easy-GoTM RT PreMix Easy-GoTM RT PreMix is a new, powerful, ready-to-use RT kit for the synthesis of cDNA with superiority to other RT products. Advantages Speed: Substantial reduction in reaction setup time. Stability: As each tube of Easy-GoTM RT PreMix contains a stabilizer that can maintain the stability of the RTase up to year at -20oC. Reproducibility and Yield: Produced in large batches, Easy-GoTM RT PreMix provides consistency over thousands of reactions. The strict functional QC assays demonstrated highly consistent and reproducible RT performance. In most applications an increase in yield is observed as compared to the standard reactions. Simplicity: The fewer manual steps allow reduction in potential errors. Each tube contains tracking dye and precipitant for agarose gel electrophoresis, eliminating the needs for a separate loading buffer. Cat. No. Description and Size FRT-2.5 Easy-GoTM RT PreMix, 25 tubes, 0.5ml thin wall microtube, 20µl reaction FRT-2.2 Easy-GoTM RT PreMix, 24 tubes, 0.2ml thin wall microtubes, 20µl reaction Easy-GoTM RT-PCR PreMix Easy-GoTM RT-PCR PreMix contains all the components necessary for cDNA synthesis and amplification in one tube. Advantages Speed: Substantial reduction in reaction setup time Stability: As each tube of Easy-GoTM RT-PCR PreMix contains a stabilizer, which can maintain the stability of the RTase and DNA polymerase up to a year at -20oC. RTase inactivation prior to PCR has no effects on the activity of the DNA polymerase. Reproducibility and Yield: The strict functional QC assays demonstrated highly consistent and reproducible RT-PCR performance. In most applications an increase in yield is observed as compared to the standard reactions. Simplicity: The fewer manual steps allow reduction in potential errors. Each tube contains tracking dye and precipitant for agarose gel electrophoresis, eliminating the needs for a separate loading buffer. Cat. No. Description and Size FRTP-2.5 Easy-GoTM RT-PCR PreMix, 25 tubes, 0.5ml thin wall microtube, 20µl reaction FRTP-2.2 Easy-GoTM RT-PCR PreMix, 24 tubes, 0.2ml thin wall microtubes, 20µl reaction RT-PCR using Easy-GoTM RT PreMix and Easy-GoTM RT-PCR PreMix Lane 1-2 cDNA synthesis using Easy-GoTM RT PreMix followed by PCR amplification (1.5kb). Land 3-4 RT-PCR using Easy-GoTM RT-PCR PreMix (1.5kb). Viswagen Biotech Pvt. Ltd., 23/863G, Thazhathuveettil Buildings, Market Road, Pala, Kerala 686 575; India Web: www.viswagen.com, Phone: +91-98-46804374; Fax: +91-4822-215075 Contacts: customer service: customer.service@viswagen.com Copyright © 2007 Viswagen Biotech Pvt. Ltd.